Adiponectin (ADP) is a hormone-like protein produced by adipose tissue, playing a key role in regulating metabolic processes. It enhances insulin sensitivity, helps prevent diabetes, reduces the risk of atherosclerosis, and exhibits anti-inflammatory properties. The Shanghai Jinma Bio Adiponectin ELISA Kit is specifically designed for scientific research purposes to quantify human adiponectin levels in various biological samples such as cell culture media, body fluids, tissues, serum, and plasma.
The ELISA method used in this kit follows a double-antibody sandwich approach. A microplate is pre-coated with purified human ADP antibodies to serve as a solid-phase carrier. During the experiment, standards and samples are added sequentially, followed by the addition of HRP-labeled ADP antibody, forming an antigen-antibody-enzyme-labeled antibody complex. After multiple washes, TMB substrate is introduced, which turns blue under the action of peroxidase. The reaction is stopped with sulfuric acid, resulting in a yellow color. The intensity of the color is directly proportional to the ADP concentration in the sample. The absorbance is measured at 450 nm using a microplate reader, and the ADP level in the sample is determined by comparing it to a standard curve.
The indirect method is commonly used in ELISA for detecting antibodies. This technique involves using an enzyme-conjugated secondary antibody to detect the primary antibody bound to the solid-phase antigen. Here's how it works:
- First, a specific antigen is immobilized on a solid support, creating a solid-phase antigen. Unbound antigens are removed through washing.
- The diluted sample is then added, allowing any specific antibodies present to bind to the solid-phase antigen, forming an antigen-antibody complex. After washing, only the bound antibodies remain on the plate.
- An enzyme-labeled secondary antibody is added, which binds to the primary antibody, thereby indirectly labeling it. Following another round of washing, the amount of enzyme on the plate correlates with the amount of target antibody in the original sample.
Shanghai Jinma Biotech offers high-quality ELISA kits at competitive prices, trusted by numerous research institutions worldwide. The Adiponectin ELISA Kit is ideal for researchers looking for accurate and reliable detection methods. For more information or to request free samples, feel free to contact us today!
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