Adiponectin (ADP) is a hormone-like protein secreted by adipose tissue that plays a crucial role in metabolic regulation. It has been shown to improve insulin sensitivity, reduce the risk of diabetes, prevent atherosclerosis, and exhibit anti-inflammatory properties. The Shanghai Jinma Bio Adiponectin ELISA Kit is designed for scientific research purposes and allows for the quantitative detection of human adiponectin in various biological samples such as cell culture supernatants, body fluids, tissues, serum, and plasma.
The kit utilizes a double-antibody sandwich ELISA method to measure ADP levels. A microplate is pre-coated with purified human ADP antibodies to serve as a solid-phase carrier. During the experiment, standards and samples are added sequentially, followed by the addition of HRP-labeled ADP antibody. This forms an immune complex of antibody-antigen-enzyme-labeled antibody. After washing, the substrate TMB is added, which turns blue under peroxidase activity. The reaction is stopped with sulfuric acid, turning the solution yellow. The intensity of the color is directly proportional to the concentration of ADP in the sample. The absorbance at 450 nm is measured using a microplate reader, and the ADP concentration is determined based on a standard curve.
The indirect ELISA method is commonly used for detecting antibodies. This approach involves the use of an enzyme-labeled secondary antibody to detect the presence of specific antibodies bound to a solid-phase antigen. The process includes the following steps:
- Coating a solid-phase carrier with a specific antigen to create a solid-phase antigen. After washing, unbound antigens and impurities are removed.
- Add the diluted sample and allow it to react. The specific antibodies in the sample bind to the solid-phase antigen, forming an antigen-antibody complex. After washing, only the bound antibodies remain on the plate.
- Add the enzyme-labeled secondary antibody. This binds to the primary antibody, indirectly labeling it with the enzyme. After washing, the amount of enzyme remaining on the plate reflects the level of target antibody in the sample.
Shanghai Jinma Biotech offers high-quality products at competitive prices. Our Adiponectin ELISA Kit ensures reliable results and is trusted by many scientific research institutions. For more information or to request free samples, feel free to contact us today!
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