(2) If one or more mice display unusual behavior or die during the test, this could indicate potential toxicity. In such cases, it is recommended to repeat the experiment using a larger group of ten mice to verify the initial findings and ensure the accuracy of the results.
(3) Whenever possible, any mouse that dies or shows severe signs of poisoning should be subjected to a post-mortem examination. This step helps identify the exact cause of death or poisoning, providing valuable insights into the effects of the gas sample on living organisms.
It is important to maintain detailed records of all observations, including behavioral changes, physical symptoms, and outcomes. These records not only support the reliability of the test but also help in future assessments or comparisons with other substances. Additionally, following proper safety protocols and ensuring ethical treatment of animals are crucial aspects of conducting such experiments.
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